CoronaMelt is a new Real Time PCR detection kit for SARS-CoV-2 virus, designed for high sensitivity detection of viral RNA in samples derived from nasopharyngeal and oropharyngeal swabs.
The assay is based on Real Time PCR amplification followed by melting curve analysis to confirm the identity of amplified product. CoronaMelt is compatible with most commercial Real Time PCR instruments having melting curve analysis capability and with Magnetic Bead or column RNA extraction methods.
The assay includes two amplicons designed on SARS-CoV-2 targets located in the ORF1ab gene and one amplicon designed on human GAPDH transcript, used as an endogenous control.
The use of an endogenous control, which is detected simultaneously with the viral targets, warrants a complete verification of the process from sample collection through transportation, RNA extraction and amplification. Bad sampling or incorrect sample storage will not generate false negative reports.
Brochure available here: